{"version":"1.0","provider_name":"Microsoft Research","provider_url":"https:\/\/www.microsoft.com\/en-us\/research","author_name":"Nicolo Fusi","author_url":"https:\/\/www.microsoft.com\/en-us\/research\/people\/fusi\/","title":"In Silico Predictive Modeling of CRISPR\/Cas9 guide efficiency - Microsoft Research","type":"rich","width":600,"height":338,"html":"<blockquote class=\"wp-embedded-content\" data-secret=\"oBYY1WeyKR\"><a href=\"https:\/\/www.microsoft.com\/en-us\/research\/publication\/in-silico-predictive-modeling-of-crisprcas9-guide-efficiency\/\">In Silico Predictive Modeling of CRISPR\/Cas9 guide efficiency<\/a><\/blockquote><iframe sandbox=\"allow-scripts\" security=\"restricted\" src=\"https:\/\/www.microsoft.com\/en-us\/research\/publication\/in-silico-predictive-modeling-of-crisprcas9-guide-efficiency\/embed\/#?secret=oBYY1WeyKR\" width=\"600\" height=\"338\" title=\"&#8220;In Silico Predictive Modeling of CRISPR\/Cas9 guide efficiency&#8221; &#8212; Microsoft Research\" data-secret=\"oBYY1WeyKR\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\" class=\"wp-embedded-content\"><\/iframe><script type=\"text\/javascript\">\n\/* <![CDATA[ *\/\n\/*! This file is auto-generated *\/\n!function(d,l){\"use strict\";l.querySelector&&d.addEventListener&&\"undefined\"!=typeof URL&&(d.wp=d.wp||{},d.wp.receiveEmbedMessage||(d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!\/[^a-zA-Z0-9]\/.test(t.secret)){for(var s,r,n,a=l.querySelectorAll('iframe[data-secret=\"'+t.secret+'\"]'),o=l.querySelectorAll('blockquote[data-secret=\"'+t.secret+'\"]'),c=new RegExp(\"^https?:$\",\"i\"),i=0;i<o.length;i++)o[i].style.display=\"none\";for(i=0;i<a.length;i++)s=a[i],e.source===s.contentWindow&&(s.removeAttribute(\"style\"),\"height\"===t.message?(1e3<(r=parseInt(t.value,10))?r=1e3:~~r<200&&(r=200),s.height=r):\"link\"===t.message&&(r=new URL(s.getAttribute(\"src\")),n=new URL(t.value),c.test(n.protocol))&&n.host===r.host&&l.activeElement===s&&(d.top.location.href=t.value))}},d.addEventListener(\"message\",d.wp.receiveEmbedMessage,!1),l.addEventListener(\"DOMContentLoaded\",function(){for(var e,t,s=l.querySelectorAll(\"iframe.wp-embedded-content\"),r=0;r<s.length;r++)(t=(e=s[r]).getAttribute(\"data-secret\"))||(t=Math.random().toString(36).substring(2,12),e.src+=\"#?secret=\"+t,e.setAttribute(\"data-secret\",t)),e.contentWindow.postMessage({message:\"ready\",secret:t},\"*\")},!1)))}(window,document);\n\/* ]]> *\/\n<\/script>\n","description":"The CRISPR\/Cas9 system provides unprecedented genome editing capabilities; however, several facets of this system are under investigation for further characterization and optimization, including the choice of guide RNA that directs Cas9 to target DNA. In particular, given that one would like to target the protein-coding region of a gene, hundreds of guides satisfy the basic [&hellip;]"}